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spr binding and competitive experiments  (Biacore)

 
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    Structured Review

    Biacore spr binding and competitive experiments
    ( A ) <t>SPR</t> sensorgrams of HMGA1a (top left panel) and HMGB1 (top right panel) binding to dsDNA biotin-GGGGAATAATCGCGATTATTCCCCAATAATCGCGATTATT in HEPES 1 <t>at</t> <t>25°C.</t> For HMGA1a, the best sensorgrams were obtained in HEPES 1 buffer, instead of MES or Phosphate buffer . ( B ) Binding curves for interaction of HMGA1a (lower left panel) and HMGB1 (lower right panel) with target DNA and fitting curve for a two-site affinity model.
    Spr Binding And Competitive Experiments, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spr binding and competitive experiments/product/Biacore
    Average 90 stars, based on 1 article reviews
    spr binding and competitive experiments - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Functional and structural analysis of AT-specific minor groove binders that disrupt DNA–protein interactions and cause disintegration of the Trypanosoma brucei kinetoplast"

    Article Title: Functional and structural analysis of AT-specific minor groove binders that disrupt DNA–protein interactions and cause disintegration of the Trypanosoma brucei kinetoplast

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkx521

    ( A ) SPR sensorgrams of HMGA1a (top left panel) and HMGB1 (top right panel) binding to dsDNA biotin-GGGGAATAATCGCGATTATTCCCCAATAATCGCGATTATT in HEPES 1 at 25°C. For HMGA1a, the best sensorgrams were obtained in HEPES 1 buffer, instead of MES or Phosphate buffer . ( B ) Binding curves for interaction of HMGA1a (lower left panel) and HMGB1 (lower right panel) with target DNA and fitting curve for a two-site affinity model.
    Figure Legend Snippet: ( A ) SPR sensorgrams of HMGA1a (top left panel) and HMGB1 (top right panel) binding to dsDNA biotin-GGGGAATAATCGCGATTATTCCCCAATAATCGCGATTATT in HEPES 1 at 25°C. For HMGA1a, the best sensorgrams were obtained in HEPES 1 buffer, instead of MES or Phosphate buffer . ( B ) Binding curves for interaction of HMGA1a (lower left panel) and HMGB1 (lower right panel) with target DNA and fitting curve for a two-site affinity model.

    Techniques Used: Binding Assay



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    spr binding and competitive experiments  (Biacore)
    90
    Biacore spr binding and competitive experiments
    ( A ) <t>SPR</t> sensorgrams of HMGA1a (top left panel) and HMGB1 (top right panel) binding to dsDNA biotin-GGGGAATAATCGCGATTATTCCCCAATAATCGCGATTATT in HEPES 1 <t>at</t> <t>25°C.</t> For HMGA1a, the best sensorgrams were obtained in HEPES 1 buffer, instead of MES or Phosphate buffer . ( B ) Binding curves for interaction of HMGA1a (lower left panel) and HMGB1 (lower right panel) with target DNA and fitting curve for a two-site affinity model.
    Spr Binding And Competitive Experiments, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spr binding and competitive experiments/product/Biacore
    Average 90 stars, based on 1 article reviews
    spr binding and competitive experiments - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) SPR sensorgrams of HMGA1a (top left panel) and HMGB1 (top right panel) binding to dsDNA biotin-GGGGAATAATCGCGATTATTCCCCAATAATCGCGATTATT in HEPES 1 at 25°C. For HMGA1a, the best sensorgrams were obtained in HEPES 1 buffer, instead of MES or Phosphate buffer . ( B ) Binding curves for interaction of HMGA1a (lower left panel) and HMGB1 (lower right panel) with target DNA and fitting curve for a two-site affinity model.

    Journal: Nucleic Acids Research

    Article Title: Functional and structural analysis of AT-specific minor groove binders that disrupt DNA–protein interactions and cause disintegration of the Trypanosoma brucei kinetoplast

    doi: 10.1093/nar/gkx521

    Figure Lengend Snippet: ( A ) SPR sensorgrams of HMGA1a (top left panel) and HMGB1 (top right panel) binding to dsDNA biotin-GGGGAATAATCGCGATTATTCCCCAATAATCGCGATTATT in HEPES 1 at 25°C. For HMGA1a, the best sensorgrams were obtained in HEPES 1 buffer, instead of MES or Phosphate buffer . ( B ) Binding curves for interaction of HMGA1a (lower left panel) and HMGB1 (lower right panel) with target DNA and fitting curve for a two-site affinity model.

    Article Snippet: SPR binding and competitive experiments were performed at 25°C with a Biacore X-100 apparatus (Biacore GE).

    Techniques: Binding Assay